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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in many tumors, which results in chromosome instability, and so may possibly lead to tumorigenesis. This protein was discovered to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important position in marketing mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been explained. Two further splice variants were described but comprehensive nucleotide sequence information and facts is just not however out there.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide generally has the epitope recognized through the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make certain the best possible success are...
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This tutorial outlines further more probable optimization here of mobile and immunoassay parameters to ensure the very best results are...
Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, one can see which staining is certain.
Goal Details Cyclin E belongs towards the extremely conserved cyclin family, whose users are characterised by a spectacular periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins show distinct expression and degradation styles which lead for the temporal coordination of each mitotic celebration. Cyclin E varieties a posh with and capabilities to be a regulatory subunit of CDK2, whose exercise is necessary for cell cycle G1/S transition. This protein accumulates within the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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